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HelpTest Code CGS Chlamydia/Gonorrhea by PCR-Swab
Methodology
Polymerase Chain Reaction (PCR)
Specimen Type
| Male | Female | Swab submitted for testing | |
| Oropharyngeal | √ | √ | Woven |
| Anorectal | √ | √ | Woven |
| Endocervical | √ | Flocked | |
| Vaginal | √ | Woven |
Preferred Collection Container
COBA-DUAL
Collection Volume
Only submit ONE swab.
For oropharyngeal, anorectal, and endocervical specimens submit woven swab.
For endocervical specimens submit flocked swab.
Minimum Collection Volume
Only submit ONE swab.
For oropharyngeal, anorectal, and endocervical specimens submit woven swab.
For endocervical specimens submit flocked swab.
Collection Instructions
| Specimen Type | Specimen Collection |
| Oropharyngeal |
Use Woven Swab.
Carefully but firmly guide swab over several areas of inflammation or pus, tonsils (or tonsilar crypes if tonsils have been removed) or posterior pharynx.
Withdraw swab carefully, avoid toughing other surfaces in mouth and insert swab into collection tube.
Break swab shaft at indicated height. Discard top portion of shaft and place bottom portion in specimen collection tube.
Mix thoroughly to expose specimen to nucleic acid stabilizing agents in transport media.
|
| Anorectal |
Use Woven Swab.
Insert swab approximately 1.5 to 2 inches (3 to 5 cm) in the rectum.
Withdraw swab carefully, avoid toughing other surfaces and insert swab into collection tube.
Break swab shaft at indicated height. Discard top portion of shaft and place bottom portion in specimen collection tube.
Mix thoroughly to expose specimen to nucleic acid stabilizing agents in transport media.
|
| Endocervical |
Requires use of a speculum and two swabs, one for initial cleaning of the cervix to remove excess mucus from the cervical os and surrounding mucosa (this swab is discarded) and a second swab for specimen collection.
Using the Woven swab, remove cervical mucous and discard this swab.
Once cervical mucous is removed, use the Flocked swab to collect the sample.
Withdraw swab carefully, avoid toughing the vaginal mucosa and insert swab into collection tube.
Break swab shaft at indicated height. Discard top portion of shaft and place bottom portion in specimen collection tube.
Mix thoroughly to expose specimen to nucleic acid stabilizing agents in transport media.
|
| Vaginal |
Use Woven Swab.
Carefully insert swab approximately 2 inches into the vaginal opening and gently turn/rub the swab against the vaginal wall.
Withdraw swab carefully, avoid toughing other surfaces and insert swab into collection tube.
Break swab shaft at indicated height. Discard top portion of shaft and place bottom portion in specimen collection tube.
Mix thoroughly to expose specimen to nucleic acid stabilizing agents in transport media.
|
See the Collection Guide
Transport
Room Temperature
Rejection Criteria
Collection container with 2 swabs; No order with specimen; Specimens not collected/transported in sterile/proper container; Specimens collected in M4RT; Specimen other than indicated; Throat, rectal or other specimen types; Improperly labelled specimen.
Specimen Storage and Stability
Room Temperature: 90 days
Refrigerated: 90 days
Turn Around Time
2-3 days. This test is routinely run Monday through Friday, except holidays.
Lab Department
Microbiology
Alias
Chlamydia DNA; Chlamydia trachomatis DNA Amplification by PCR; Gonorrhoeae DNA; Neisseria gonorrhoeae DNA Amplification by PCR; Gonorrhea; Gonorrhoea; GC; CT
Ordering Info
Test not appropriate on patients <14 years old. See alternate test CTGTMA on urine only.
INCLUDES: Chlamydia trachomatis and Neisseria gonorrhoeae PCR testing.
EPIC Code
LAB245A
Additional Information
Limitations: Results are dependent upon adequate specimen collection and proper transport conditions. The following are known PCR inhibitors: Replens lubricant, excess mucous. It is important to remove cervical secretions and discharge before obtaining the sample for testing. Also, samples containing greater than 10%(v/v) blood are unacceptable.
Day(s) and Time(s) Performed
Monday through Friday, 8:00am – 4:30pm
STAT Availability
No