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Test Code CHMF8 Chromogenic Factor 8 Activity Assay

Important Note

In house testing down. See specimen requirements for Mayo's test CHF8

Specimen Type

Blood.

Preferred Collection Container

BLUE

Collection Volume

2.7 mL in 2.7 mL tube

Minimum Collection Volume

2.7 mL in 2.7 mL tube

Collection Instructions

Routine venipuncture. If multiple tests are being drawn, the recommended order of draw is as follows: blood cultures, sodium citrate tubes, gel separator/plain serum plastic tubes, heparin tubes, EDTA tubes. A special tube should be obtained from the laboratory for hematocrits over 55%. Specimens should be transported as soon as possible to the Clinical Laboratory.

Rejection Criteria

Specimens that are clotted, hemolyzed, diluted with IV fluid, received unfrozen more than 4 hours after collection, insufficient volume, improperly labelled, not double spun (not platelet poor).

Specimen Storage and Stability

Freeze double spun aliquots within 4 hours after collection. Samples may be frozen at -20° C for up to 2 weeks or at -70° C for up to six months.

Turn Around Time

24 hours.

Lab Department

Hematology

Ordering Info

2-stage chromogenic assay that should be performed to diagnose Hemophilia A and to monitor FVIII replacement therapy.

EPIC Code

LAB306A

Acceptable Collection Container

BLUE (3.2% buffered sodium citrate)

Transfer Instructions

Centrifuge samples at 1500 g for 15 minutes. Remove plasma with plastic transfer pipette and place into a plastic tube. Centrifuge the transferred plasma at 1500 g for 15 minutes. Transfer plasma to new plastic tubes (1 mL aliquots per tube) and freeze.

Day(s) and Time(s) Performed

Monday through Friday, 7am to 3pm

STAT Availability

Yes-must call Hematology to make arrangements