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HelpTest Code F2 Factor 2 Assay
Specimen Type
Blood.
Preferred Collection Container
BLUECollection Volume
2.7 mL in 2.7 mL tube
Minimum Collection Volume
2.7 mL in 2.7 mL tube
Collection Instructions
Routine venipuncture. If multiple tests are being drawn, the recommended order of draw is as follows: blood cultures, sodium citrate tubes, gel separator/plain serum plastic tubes, heparin tubes, EDTA tubes. A special tube should be obtained from the laboratory for hematocrits over 55%. Specimens should be transported as soon as possible to the Clinical Laboratory.
Rejection Criteria
Specimens that are clotted, hemolyzed, diluted with IV fluid, received unfrozen more than 4 hours after collection, insufficient volume, improperly labelled, not double spun (not platelet poor).
Specimen Storage and Stability
Freeze double spun aliquots within 4 hours after collection. Samples may be frozen at -20° C for up to 2 weeks or at -70° C for up to six months.
Turn Around Time
24 hours.
Lab Department
Hematology
Alias
Prothrombin Assay; Factor 2 Assay; Factor II Assay;
Ordering Info
NOTE: Collection of blood for coagulation testing through indwelling catheters that have been flushed with heparin should be avoided, if possible. If specimen is drawn through an indwelling catheter, the line should be flushed with 5 ml saline and the first 5 ml of blood or six times the deadspace volume of the catheter being used should be discarded. Proper collection and handling of specimens is critical.EPIC Code
LAB303
Pediatric Collection Container
BLUEAcceptable Collection Container
BLUE (3.2% buffered sodium citrate)
Transfer Instructions
Centrifuge samples at 1500 g for 15 minutes. Remove plasma with plastic transfer pipette and place into a plastic tube. Centrifuge the transferred plasma at 1500 g for 15 minutes. Transfer plasma to new plastic tubes (1 mL aliquots per tube) and freeze.
Day(s) and Time(s) Performed
Monday through Friday, 7am to 3pm
STAT Availability
Yes-must call Hematology to make arrangements